目的 探讨大理不同产地多叶重楼和云南重楼根茎HPLC指纹图谱及7种主要甾体皂苷成分差异。方法 采用高效液相色谱法建立大理不同产地多叶重楼和云南重楼根茎HPLC指纹图谱,并对指纹图谱相似度及7种主要甾体皂苷成分进行比较分析。结果 大理不同产地多叶重楼根茎HPLC指纹图谱有14个共有峰,除巍山金华、巍山龙街外其他产地多叶重楼根茎HPLC指纹图谱相似度均大于0.9;大理不同产地云南重楼根茎HPLC指纹图谱有13个共有峰,大理不同产地云南重楼根茎HPLC指纹图谱相似度均大于0.92;大理不同产地多叶重楼和云南重楼根茎HPLC指纹图谱有11个共有峰,大理不同产地多叶重楼和云南重楼根茎HPLC指纹图谱相似度极低,相似度仅在0.057~0.225之间。大理不同产地多叶重楼和云南重楼根茎中均检测到重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅵ和重楼皂苷Ⅶ,大理不同产地云南重楼根茎中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅵ和重楼皂苷Ⅶ的峰面积均值高于多叶重楼,在南涧乐秋、巍山金华、洱源凤羽、洱源邓川、弥渡红岩、鹤庆西邑多叶重楼和大理湾桥、大理凤仪、鹤庆西邑、弥渡红岩、南涧乐秋云南重楼根茎中均检测到重楼皂苷H,除巍山龙街外其他产地多叶重楼根茎中检测到重楼皂苷Ⅲ,在洱源凤羽、鹤庆西邑多叶重楼根茎中还检测到重楼皂苷Ⅴ。结论 大理不同产地多叶重楼和云南重楼根茎中均检测到重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅵ和重楼皂苷Ⅶ,多叶重楼和云南重楼根茎HPLC指纹图谱相似度极低,7种主要甾体皂苷成分也存在较大差异,大理不同产地云南重楼根茎中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅵ和重楼皂苷Ⅶ的含量高于多叶重楼。
Abstract
OBJECTIVE To study the fingerprints of rhizomes of Paris polyphylla Smith var. polyphylla and Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz from different origins in Dali and the differences of seven main steroidal saponins. METHODS The fingerprints of rhizomes of Paris polyphylla var. polyphylla and Paris polyphylla var. yunnanensis from different origins in Dali were established by HPLC. The similarity of fingerprints and seven main steroidal saponins were compared and analyzed. RESULTS There were 14 common peaks in the fingerprints of rhizomes of Paris polyphylla var. polyphylla from different origins in Dali, and the similarity of the fingerprints of the samples from different habitats except Jinhua Weishan and Longjie Weishan was greater than 0.9. There were 13 common peaks in the fingerprints of rhizomes of Paris polyphylla var. yunnanensis from different origins in Dali, and the similarity of the samples from different origins was greater than 0.92. There were 11 common peaks in the fingerprints of rhizomes of Paris polyphylla var. polyphylla and Paris polyphylla var. yunnanensis from different origins in Dali, and the similarity of the fingerprints was very low, between 0.057 and 0.225. Polyphyllin Ⅰ, polyphyllin Ⅱ, polyphyllin Ⅵ and polyphyllin Ⅶ are detected in the rhizomes of Paris polyphylla var. polyphylla and Paris polyphylla var. yunnanensis from different origins in Dali. The average peak areas of polyphyllin Ⅰ, polyphyllin Ⅱ, polyphyllin Ⅵ and polyphyllin Ⅶ in Paris polyphylla var. yunnanensis from different origins in Dali were higher than those of Paris polyphylla var. polyphylla. Polyphyllin H was detected in the rhizomes of Paris polyphylla var. yunnanensis from Leqiu Nanjian, Jinhua Weishan, Fengyu Eryuan, Dengchuan Eryuan, Hongyan Midu, Xiyi Heqing and the rhizomes of Paris polyphylla var. polyphylla from Wanqiao Dali, Fengyi Dali, Xiyi Heqing, Hongyan Midu and Leqiu Nanjian. Polyphyllin Ⅲ was detected in the rhizomes of Paris polyphylla var. polyphylla from the origins except Longjie Weishan. Polyphyllin Ⅴ was also detected in the rhizomes of Paris polyphylla var. polyphylla in Fengyu Eryuan and Xiyi Heqing. CONCLUSION Polyphyllin Ⅰ, polyphyllin Ⅱ, polyphyllin Ⅵ and polyphyllin Ⅶ are detected in the rhizomes of Paris polyphylla var. polyphylla and Paris polyphylla var. yunnanensis from different origins in Dali. The similarity of the HPLC fingerprints of the rhizomes of Paris polyphylla var. polyphylla and Paris polyphylla var. yunnanensis is very low, and there are great differences in seven main steroidal saponins. The contents of polyphyllin Ⅰ, polyphyllin Ⅱ, polyphyllin Ⅵ and polyphyllin Ⅴ in Paris polyphylla var. yunnanensis are higher than those in Paris var. polyphylla.
关键词
多叶重楼 /
云南重楼 /
HPLC指纹图谱 /
主成分 /
甾体皂苷 /
重楼皂苷Ⅰ /
重楼皂苷Ⅱ
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Key words
Paris polyphylla Smith var. polyphylla /
Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz /
HPLC fingerprint /
principal component /
steroidal saponin /
polyphyllin Ⅰ /
polyphyllin Ⅱ
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金项目资助(81360616)
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